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Pakistan Journal of Biological Sciences

Year: 2008  |  Volume: 11  |  Issue: 24  |  Page No.: 2675 - 2678

The First Report of SVC from Indian Carp Species by PCR and Histopathologic Methods in Iran

A. Haghighi-Khiabanian Asl, M. Azizzadeh, M. Bandehpour, Z. Sharifnia and B. Kazemi

Abstract

In this research, we confirmed the positive SVC in three Indian carps spp. (1) Rohu (Labeo rohita), (2) Merigal (Cirrhinus merigala) and (3) Catla (Catla catla) with typical histopathologic signs and PCR sequencing. Nested-PCR used to amplify a fragment of viral glycoprotein gene in Shahid Beheshti University M.C. and PCR product was purified and submitted to sequencing and deposited to GenBank at accession No. FJ711168. Two sites in this research that, were placed in Khuzestan Province (Ahvaz City, South of Iran) and Gilan Province (Astaneh Ashrafieh City, North of Iran) aquaculture farms. Samples were prepared for PCR method in both sites (30 pieces from Khuzestan and 30 pieces from Gilan Province), that all of the (100%) samples were positive in Nested-PCR. In addition, other 60 samples for histopathologic studies in both sites (30 pieces from Khuzestan and 30 pieces from Gilan Province), (according to 10% prevalence). All of the (100%) typically samples were with major histopathologic signs. The results of this study indicate that SVC infection can be found in some Indian carp in Iran.

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