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Pakistan Journal of Biological Sciences

Year: 2008  |  Volume: 11  |  Issue: 24  |  Page No.: 2660 - 2664

Designing and Introduce a Diagnostic Kit for Detection of White Spot Syndrome Virus in Cultured Penaeus indicus in Iran

A.M. Saberi, M. Bandehpour, M. Afsharnasab, E. Ghayour, S.A. Yousefi Namin and B. Kazemi

Abstract

The aim of this study is designing a diagnostic kit for white spot syndrome virus. We designed 2 series of primers for diagnosis of viral VP24 gene and also primers as internal controls which amplify part of genome in both positive and negative samples. DNA of shrimps were extracted and PCR amplification carried out. In this research, a diagnosis kit for white spot disease of shrimps designed and tested using 32 shrimp samples which were dubious to have this disease. White spot virus were found in 23 samples and the other 9 were negative. For extra confirmation, the PCR product was sequenced and deposited to GenBank. We designed a diagnosis kit for white spot disease of shrimps and tested successfully.

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