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Pakistan Journal of Biological Sciences
Year: 2008  |  Volume: 11  |  Issue: 24  |  Page No.: 2653 - 2659

Tetraploid Induction of Hyoscyamus muticus L. using Colchicine Treatment

F. Shahriari-Ahmadi, E. Dehghan, M. Farsi and M. Azizi    

Abstract: In this study the tip of meristem in diploid plants (2n = 2x =28) were treated with a 0.2% colchicine solution and flowcytometry was used to determine the DNA ploidy level of different tissues in individual plants. Morphological characteristics such as size and thickness of leaves, flowers, seeds, epidermis and guard cells, as well as the number of chloroplast in guard cells were considered as other parameters for identifying putative tetraploid plants. We show that, the DNA ploidy of Hyoscyamus muticus, treated with Colchicine can be evaluated using flow cytometry. In addition we also report reliable morphological markers to identify putative tetraploids in this species. Although, tetraploidy was induced using this technique, mixoploid plants were also observed among the Egyptian henbane plants. The results of this study also indicate that colchicine treatment can efficiently be used to improve Hyoscyamus muticus for commercial and pharmaceutical applications of its vegetative organs.

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