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Pakistan Journal of Biological Sciences
Year: 2008  |  Volume: 11  |  Issue: 19  |  Page No.: 2336 - 2340

Removal of Headspace CO2 Increases Biological Hydrogen Production by C. acetobutylicum

H. Alshiyab, M.S. Kalil, A.A. Hamid and W.M.W. Yusoff    

Abstract: The effect of removal of resultant gas resulted in enhancement of the H2 yield. The technique of CO2 scavenging resulted in H2 yield being improved from 408 mL g-1 to reach the maximum of 422 mL g-1. The highest hydrogen productivity of 87.9 ml L-1 h-1 was obtained by CO2 scavenging. Biomass concentration was enhanced to 1.47 g L-1, YP/X of 287 ml g-1 L-1, YX/S of 0.294 and YH2/s of 0.0377 by the use of CO2 scavenging. The results suggested that the presence of the gaseous products in fermentation medium and headspace adversely effect biomass growth and hydrogen production.

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