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Pakistan Journal of Biological Sciences

Year: 2008  |  Volume: 11  |  Issue: 12  |  Page No.: 1638 - 1641

The Influence of Different Hormone Concentration and Combination on Callus Induction and Regeneration of Rauwolfia serpentina L. Benth

U. Salma, M.S.M. Rahman, S. Islam, N. Haque, T.A. Jubair, A.K.M.F. Haque and I.J. Mukti

Abstract

The influence of media composition on callus induction and subsequent regeneration of Rauwolfia serpentina L. Benth has been studied. High frequency (96.43%) callus induction was obtained when nodal segments from in vitro raised shoots were cultured on MS medium supplemented with 0.5 mg L-1 BA and 2.0 mg L-1 NAA. The callus differentiated into adventitious shoots when it was subcultured on MS medium supplemented with 2.0 mg L-1 BA with 0.2 mg L-1 NAA. Regenerated shoots were best rooted on half-strength MS medium with 1.0 mg L-1 each of IBA and IAA.

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