Mass Propagation of Rauwolfia serpentina L. Benth
A protocol for mass propagation through axillary bud proliferation was
established for Rauwolfia serpentina L. Benth. (Apocynaceae). MS
medium supplemented with 1.5 mg L-1 BA and 0.2 mg L-1
NAA elicited the maximum number of shoots (4 multiple shoots) from nodal
explants. These adventitious shoots were best rooted on half strength
MS medium supplemented with 1.0 mg L-1 each of IBA and IAA.
The in vitro raised plants were acclimatized in glass house and
successfully transplanted to field condition with almost 95% survival.
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