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Pakistan Journal of Biological Sciences

Year: 2008  |  Volume: 11  |  Issue: 9  |  Page No.: 1273 - 1277

Mass Propagation of Rauwolfia serpentina L. Benth

U. Salma, M.S.M. Rahman, S. Islam, N. Haque, M. Khatun, T.A. Jubair and B.C. Paul


A protocol for mass propagation through axillary bud proliferation was established for Rauwolfia serpentina L. Benth. (Apocynaceae). MS medium supplemented with 1.5 mg L-1 BA and 0.2 mg L-1 NAA elicited the maximum number of shoots (4 multiple shoots) from nodal explants. These adventitious shoots were best rooted on half strength MS medium supplemented with 1.0 mg L-1 each of IBA and IAA. The in vitro raised plants were acclimatized in glass house and successfully transplanted to field condition with almost 95% survival.

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