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Pakistan Journal of Biological Sciences
Year: 2007  |  Volume: 10  |  Issue: 22  |  Page No.: 4015 - 4021

Detection of Legionella pneumophila by PCR-ELISA Method in Industrial Cooling Tower Water

Soheili Majid, Nejadmoghaddam Mohammad Reza, Babashamsi Mohammad, Ghasemi Jamileh and Jeddi Tehrani Mahmood    

Abstract: Water supply and Cooling Tower Water (CTW) are among the most common sources of Legionella pneumophila (LP) contamination. A nonradio active method is described to detect LP in industrial CTW samples. DNA was purified and amplified by nested -PCR with amplimers specific for the 16s rRNA gene of LP. The 5 end biotinylated oligomer probe was immobilized on sterptavidin B coated microtiter plates. The nested-PCR product was labeled with digoxigenin and then hybridized with 5-biotinylated probes. The amplification products were detected by using proxidase-labled anti dioxygenin antibody in a colorimetric reaction. The assay detected LP present in 1 L of 5 CTW samples examined. All of the samples were Legionella positive in both culture and PCR-ELISA methods. The PCR-ELISA assay appears to exhibit high specificity and is a more rapid technique in comparison with bacterial culture method. Thus could prove suitable for use in the routine examination of industrial CTW contamination.

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