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Pakistan Journal of Biological Sciences

Year: 2007  |  Volume: 10  |  Issue: 17  |  Page No.: 2868 - 2873

Genetic Variation of Jointed Goatgrass (Aegilops cylindrica Host.) From Iran Using RAPD-PCR and SDS-PAGE of Seed Proteins

M. Farkhari, M.R. Naghavi, S.A. Pyghambari and Sabokdast

Abstract

Genetic variation of 28 populations of jointed goatgrass (Aegilops cylindrica Host.), collected from different parts of Iran, were evaluated using both RAPD-PCR and SDS-PAGE of seed proteins. The diversity within and between populations for the three-band High Molecular Weight (HMW) subunits of glutenin pattern were extremely low. Out of 15 screened primers of RAPD, 14 primers generated 133 reproducible fragments which among them 92 fragments were polymorphic (69%). Genetic similarity calculated from the RAPD data ranged from 0.64 to 0.98. A dendrogram was prepared on the basis of a similarity matrix using the UPGMA algorithm and separated the 28 populations into two groups. Confusion can happen between populations with the same origin as well as between populations of very diverse geographical origins. Our results show that compare to seed storage protein, RAPD is suitable for genetic diversity assessment in Ae. cylindrica populations.

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