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Pakistan Journal of Biological Sciences
Year: 2006  |  Volume: 9  |  Issue: 1  |  Page No.: 48 - 53

Regeneration of Eggplant Through Anther Culture

F. Khatun, M.B. Meah and K.M. Nasiruddin    

Abstract: The study was conducted to develop a protocol for anther culture of eggplant. Six eggplant cultivars viz., Dohazari, IPM 31, Laffa S, Ishurdi L, ISD-006 and Jessore L were implanted on MS medium with different hormone supplements viz., 2 mg L-1 NAA + 2 mg L-1 BAP, 1 mg L-1 IAA + 2 mg L-1 BAP and 2 mg L-1 2, 4-D + 2 mg L-1 BAP for callus induction. The highest survivability of anthers (35 %) and the highest percentage of callus induction (30%) were recorded in cv. ISD-006 with 2 mg L-1 NAA + 2 mg L-1 BAP supplemented MS medium. The minimum days (27.25) required for callus initiation in the cultivar ISD-006 on MS medium with hormone supplement 2 mg L-1 2-4,D + 2 mg L-1 BAP. Anther derived calli of eggplant cultivars were transferred to MS medium with various combinations and concentrations of auxin and cytokinin for shoot regeneration but the calli did not produce any shoot. Root formation was observed from the cultured calli. For different parameters in root formation, the cultivar ISD-006 showed the best performance followed by Jessore L. Among the media, MS medium with 2 mg L-1 NAA +2 mg L-1 BAP and MS medium with 2.5 mg L-1 NAA +2 mg L-1 BAP showed better performance with insignificant variation.

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