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Pakistan Journal of Biological Sciences

Year: 2006  |  Volume: 9  |  Issue: 12  |  Page No.: 2261 - 2266

Isolation and Biological Activities of Chemical Constituents from the Stems of Ipomoea turpethum

Md. Harun-Or-Rashid, Nurul Karim, M.A. Gafur, Md. Golam Sadik, A.S.M. Anisuzzaman, Noaki Sugimoto and A.T.M. Zafrul Azam

Abstract

Extensive chromatographic separation and purification of the organic solvent extracts of stem of I. turpethum afforded three compounds, N-p-coumaryl tyramine (EH4), daucosterol (CH1) and 2-hydroxybenzoic acid (CH2); first isolated from this plant. The structures of these compounds were determined by spectroscopic analysis, including 1H- and 13C-NMR, as well as by UV, IR and electrospray ionization mass spectrometry (ESI-MS). On screening against pathogenic bacteria and fungi; compounds CH2 and EH4 as well as extracts showed significant antibacterial and antifungal activity. The Minimum Inhibitory Concentration (MIC) against Bacillus subtilis and Shigella dysenteriae of compounds CH2 and EH4 were 64 μg mL-1. On in vivo cytotoxicity studies against brine shrimp; compounds CH2 and EH4 showed LC50 (Leathal concentration for 50% mortality) values 54.03 and 43.41 μg mL-1, respectively.

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