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Pakistan Journal of Biological Sciences

Year: 2006  |  Volume: 9  |  Issue: 8  |  Page No.: 1430 - 1437

Production of Cellullolytic Enzymes by a Newly Isolated, Trichoderma sp. FETL c3-2 via Solid State Fermentation Grown on Sugar Cane Baggase: Palm Kernel Cake as Substrates

Pang Pei Kheng, Darah Ibrahim, Laszlo Poppe, George Szackacs and Ibrahim Che Omar

Abstract

The production of cellulase by a local isolate Trichoderma sp. FETL c3-2 via solid state fermentation system using sugar cane baggase:palm kernel cake as substrate mixture was investigated. The optimized Solid State Fermentation (SSF) system consists of 5 g of sugar cane baggase: palm kernel cake, moisture content of 75% (w/w), pH of moistening agent of pH 7.0, at 30°C and inoculum size of 1 x 108 spores mL-1. The SSF system was also supplemented with 4% (w/w) dextrin and 6% (w/w) of yeast extract as additional carbon and nitrogen sources, respectively. Cellulose at the concentration of 0.2% (w/w) was found to be a significant inducer for cellulase production. Using the optimized SSF system, maximum FPase and CMCase production of 3.3 U g-1 substrate and 18.05 U g-1 substrate, respectively, after 4 days of fermentation time at 30°C were obtained. The modification of the SSF system resulted a 105% increment of FPase and 63% of CMCase production. Although no significant increase in growth after modification was observed, the results obtained indicated that the cellulase production by Trichoderma sp. FETL c3-2 is growth dependent.

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