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Pakistan Journal of Biological Sciences

Year: 2006  |  Volume: 9  |  Issue: 7  |  Page No.: 1320 - 1323

Plant Regeneration from Axillary Shoots Derived Callus in Aristolochia indica Linn. an Endangered Medicinal Plant in Bangladesh

N.A. Siddique, M.A. Bari, M.M. Pervin, N. Nahar, L.A. Banu, K.K. Paul, M.H. Kabir, A.K.M.N. Huda, K.M.K.B. Ferdaus and M.J. Hossin


A procedure for rapid in vitro propagation of the aromatic and medicinal plant Aristolochia indica Linn. (Family: Aristolochiaceae) from axillary shoots is described. The highest percentage of callus induction was (95.00) on Murashige and Skoog (15) (MS) medium supplemented with 2.0 mg L-1 Kn and 1.0 mg L-1 BAP. Colour of the calli were mostly light green to dark green. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented with BAP and Kn alone or in BAP combination with NAA and IAA or NAA, IAA and BAP in combination with Kn. The Highest percentage (95.00) of shoot regeneration was obtained in MS medium fortified with 2.5 mg L-1 Kn and 1.0 mg L-1 BAP. The elongated shoots developed roots on a medium containing 1 mg L-1 Kn. The rooted plants were transferred to soil.

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