Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Pakistan Journal of Biological Sciences
Year: 2006  |  Volume: 9  |  Issue: 6  |  Page No.: 1128 - 1131

Cloning and Overexpression of rplL Gene of Brucella abortus in Escherichia coli

Gholamreza Zarrini, Zargham Sepehrizadeh, Mojtaba Tabatabaei Yazdi, Qorbane Behzadiyan Nejad and Zuhair Muhammad Hassan    

Abstract: Brucella abortus ribosomal L7/L12 protein is a 12 kDa protein encoded by rplL. This gene was amplified by PCR from B. abortus S19 and cloned in pET16b expression vector. The construct was transformed in Escherichia coli BL21 (DE3) PlysS. The expression was performed by addition 1 mM IPTG (isopropyl-beta-D-thiogalacto- pyranoside) in the medium with OD = 0.6 and incubation continued for 1, 2, 4 and 8 h at 37°C. The highest production (23 mg L-1) level was achieved after 4 h incubation of induction. The recombinant protein was purified by Ni-NTA column and characterized by SDS-PAGE.

Cited References   |    Fulltext    |   Related Articles   |   Back
   
 
 
 
  Related Articles

 
 
 
 
 
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility