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Pakistan Journal of Biological Sciences
Year: 2005  |  Volume: 8  |  Issue: 7  |  Page No.: 982 - 988

Influence of pH on the ATPase Activity and Solubility of Macrobrachium rosenbergii and Penaeus monodon Muscle Myofibrillar Protein

Md. Kamal, Md. Moshiur Rahman, Mohammad Abu Jafor Bapary, Lubna Yasmin and Md. Nurullah    

Abstract: The influence of pH on the changes in myofibrillar remaining ATPase activities of M. rosenbergii and P. monodon was studied after storage at -20°C for 2 days, 0°C for 2 days and 35°C for 30 min. In all the three storage conditions, ATPase activities for both samples were lower in acidic and alkaline pH regions and the activity remains relatively high of 0.403 ° mol Pi/min mg at pH 8.1. The influence of pH on the remaining Mg2+-ATPase activity and Ca-sensitivity of M. rosenbergii and P. monodon were evaluated after storage at 0°C for 2 days and -20°C for 2 days. Mg2+-ATPase activities both in presence and absence of Ca2+ remain high at neutral pH compared to those of acidic and alkaline region. Ca-sensitivity were also very high (about 38 to 50% at pH ranges from 7.4 to 8.3) in neutral pH region where the sensitivity declined sharply both in acidic and alkaline pH region. The influence of pH on the changes in solubility of M. rosenbergii muscle myofibrils during ice and frozen storage was investigated. The highest solubility of 83 to 84% was obtained from myofibrils in the range of pH 7.4 to pH 7.9 after storage for 2 days in ice and -20°C for 2 days in frozen storage. The solubility decreased gradually both in acidic and alkaline pH regions. In the case of P. monodon, the maximum solubility of 84% were obtained at pH 7.9 after 2 days of storage at 0°C. Similarly the maximum solubility of 79% were obtained at same pH of 7.8 in myofibrils after storage at -20°C for 2 days. The solubility of P. monodon myofibrils was quite high in a wide range of pH from 6.8 to 8.3 and the solubility decreased gradually outside of these pH ranges. Comparatively higher solubility of 84% in a wide range of pH in myofibrils stored at 0°C compared to that of frozen storage also indicates the combined effect of pH and frozen storage on the denaturation of muscle myofibrils.

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