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Pakistan Journal of Biological Sciences
Year: 2005  |  Volume: 8  |  Issue: 2  |  Page No.: 284 - 286

Correlation Between Microscopic Examination and Culture for Detection and Differentiation of Mycobacterial Isolates from Cattle in the Sudan

S.H. Manal, M.E. Hamid, I.M. El Jalii and A.S. Ali    

Abstract: One hundred and sixty seven caseated lymph nodes and tuberculous lungs were collected from cattle slaughtered in various locations in Khartoum State (Sudan) and examined bacteriologically. Microscopic examination using Zeilh-Neelsen stain and culture on Lowenstein-Jensen (L-J) medium were carried out to detect and differentiate between the mycobacterial species in the specimens. Thirty-five, 35 (20.96%) samples were found to harbor acid-fast bacteria when examined microscopically. Out of the 35 acid-fast bacteria, 22 (62.86%) showed branching filaments and were identified as Mycobacterium farcinogenes. The remaining 13(37.14%) were bacilli and identified as Mycobacterium bovis. The 35 specimens that proved to harbor acid fast bacteria were cultured on L-J medium. None of the 22 specimens with branching filaments (Mycobacterium farcinogenes) grew on L-J medium when incubated aerobically at 37 ° C between four and eight weeks. 12 (92.31%) of the 13 bacilli (Mycobacterium bovis) showed visible growth using the above growth conditions. In conclusion, microscopic examination can only detect acid fast organisms in the clinical samples whereas culture on L-J medium can differentiate between acid-fast mycobacterial species.

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