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Pakistan Journal of Biological Sciences

Year: 2005  |  Volume: 8  |  Issue: 1  |  Page No.: 141 - 145

Recurrent Somatic Embryogenesis and Twin Embryo Production in Cotton

Syed Sarfraz Hussain, Tayyab Husnain and S. Riazuddin

Abstract

Current approaches of cotton improvement include the use of genetic engineering, but progress in this area is limited because of notoriously recalcitrant nature of most elite cotton cultivars in tissue culture. A well-established regeneration system is desired for the improvement of cotton through genetic engineering. As it is reported that somatic embryos have been obtained from the regenerable lines of Coker 312 and Coker 315 but the problem with these varieties was the loss of embryogenic nature of callus with the passage of time. A procedure of recurrent somatic embryogenesis and twin embryo production in Gossypium hirsutum L Cv. Coker 312JS is being reported for the first time from the older somatic embryos. Calli were subcultured regularly. Embryogenic capacity was remained stable for more than two years. Similarly, twin embryo production was seen in second cycle of this system. This will help to propagate embryos for the development seed technology and gene transfer system.

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