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Pakistan Journal of Biological Sciences

Year: 2005  |  Volume: 8  |  Issue: 9  |  Page No.: 1298 - 1302

In vitro Shoot Multiplication and Rooting of a Dessert Banana (Musa sp cv. Anupom)

M.Z. Rahman, M.H. Rahman, M.U. Mullah, N. Nahar, R.S. Sultana, M.A. Bari and M. Hossain

Abstract

The effects of various cytokinins (viz., BAP and KIN), auxins (viz., IBA, NAA and IAA) and coconut water (CW) alone or in combinations on shoot multiplication of banana cv. Anupom were investigated. The rate of multiplication varied in different treatments. Among the treatments, MS medium supplemented with 5.0 mg L-1 each of BAP + KIN and 13% coconut water produced the highest number of shoot per explant (5.8±0.154). The number of shoots responded for rooting and their survivability were found higher with IBA than NAA and IAA. MS medium supplemented with 1.0 mg L-1 IBA produced the highest number of roots per shoot (7.±0.245). Plantlets grown without any auxin in the medium gave the least number of roots/shoot (2.60±0.219). The rooted plantlets were successfully transferred in the polybags and finally were well established in the field.

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