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Pakistan Journal of Biological Sciences

Year: 2005  |  Volume: 8  |  Issue: 7  |  Page No.: 1025 - 1029

Bacteria Killing Kinetics of the Four Plant Hormones

M.H. Morshed, M. Shamim Hossain, M. Ibrahim, M.Z. Shafique, M.O.H. Helali, A. Samad, M.S. Islam and M.A. Islam

Abstract

The aim of the present study was to evaluate the antibacterial characteristic of the four synthetic plant hormones Indoleacetic acid (IAA), 2,4- dichlorophenoxyacetic acid (2,4-D), Naphthaleneacetic acid (NAA) and Gibberellic acid (GA). Antibacterial potency was assessed by measuring the zone of inhibitions on semi-solid nutrient agar bacterial inoculating petri dishes. NAA and IAA gave potent antibacterial activity at concentration of 150 μg disc-1 giving zones at 26-35 mm. Bacteriostatic and bactericidal concentrations against the pathogens were determined by serial dilution technique. Bacteriocidal concentrations of the tested principles were found to be significantly higher than their respective bacteriostatic concentrations. Minimum inhibitory concentrations that are bacteriostatic concentrations of the tested compounds were found at 32-128 μg mL-1 whereas, bactericidal concentrations established at 256-512 μg mL-1 against eight pathogenic bacteria Streptococcus -haemolyticus, Staphylococcus aureus, Pseudomonas aeroginosa, Shigella dysenteriae, Shigella flexneri, E. coli, Salmonella typhi and Klebsiella pneumoniae.

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