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Pakistan Journal of Biological Sciences
Year: 2004  |  Volume: 7  |  Issue: 11  |  Page No.: 2005 - 2008

Study on Antibiotic Resistance by Pathogenic Bacteria Isolated from Clinical Specimen

M.K.U. Parvez, M.A. Hakim, D.K. Chowdhury and M.S. Rahman    

Abstract: An attempt was made to isolate and study the resistance of bacterial pathogens from clinical specimens (stool, blood and skin lesion) of hospitalized patients against three antibiotics viz., erythromycin, tetracycline and penicillin G. Seven different strains of pathogenic bacteria viz., Salmonella typhi, S. paratyphi, A. shigella dysenteriae, S. sonnei, Staphylococcus aureus, Enterobacter aerogenes and Escherichia coli were isolated. All the isolates were found resistance to penicillin G and tetracycline except S. typhi and S. sonnei which were found sensitive to tetracycline. On the other hand, all the isolates were found to be sensitive against erythromycin tested herein and the highest zone of inhibition (26 mm) was recorded against S. typhi. Subsequent agarose gel electrophoresis showed no plasmid-DNA band in the gel indicating that observed resistance was chromosomal gene-mediated.

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