Abstract: The experiment was conducted to compare the relative efficiency of homo and heterospermic bull semen in terms of preservation quality. Spermatozoa from three different breeds of bull namely Holstein Friesian (HF), Red Chittagong (RC) and Sahiwal (SL) were mixed in equal number and preserved for 3 days. The quality of semen in terms of mass motility, normal and live sperm content of homo and heterospermic semen were studied at various preservation periods. In total 312 samples were included in the analysis. The average (%) mass motility, normal and live sperm of homospermic semen were 51.77 ± 0.49, 77.55 ± 0.45 and 78.73 ± 0.44 respectively and for heterospermic semen the corresponding values were 59.94 ± 0.85, 83.55 ± 0.78 and 83.69±0.76. The significantly (p<0.001) highest mass motility, normal and live sperm percentages were observed in heterospermic semen as compared to homospermic semen. The quality of semen between homo and heterospermic semen in terms of mass motility, normal and live sperm percentage did not differ significantly (p>0.05) between groups at first day but differed significantly (p<0.001) at second and third day of preservation. Mass motility of homo and heterospermic semen at first day were 60.77±0.55 and 62.31±0.95%, respectively. The corresponding values at third day were 44.04 ± 0.44 and 57.12 ± 0.77%. Normal sperm of homo and heterospermic semen at first day were 86.50 ± 0.43 and 86.31 ± 0.74%, respectively. The corresponding values at third day were 70.36 ± 0.38 and 81.00 ± 0.66%. Live sperm of homo and heterospermic semen at first day were 86.56 ± 0.43 and 86.54 ± 0.75%, respectively. The corresponding values at third day were 71.54 ± 0.46 and 81.42 ± 0.79%. From the above results, it was concluded that heterospermic semen could be better preserved in terms of mass motility, normal and live sperm percentage compared to homospermic ones.