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Pakistan Journal of Biological Sciences
Year: 2004  |  Volume: 7  |  Issue: 10  |  Page No.: 1808 - 1813

Purification and Characterization of Lectins from Mulberry Seeds (Morus alba L.)

M.A. Hossain, S.M. Rafiqul Islam and N. Absar    

Abstract: Three electrophoretically homogeneous lectins were purified from the extract of mulberry seeds. The two lectins i.e. MSL-2 and MLS-3 were purified by gel filtration of 100% ammonium sulfate saturated crude extract followed by ion-exchange chromatography on DEAE-cellulose while another MSL-1 was purified by further chromatography on CM-cellulose column. The purity of the lectins was checked by polyacrylamide disc gel electrophoresis. The molecular masses of MSL-1, MSL-2 and MLS-3 as determined by gel filtration were estimated to be 175, 120 and 89 kDa, respectively. Again molecular masses of MSL-1, MSL-2 and MSL-3 as determined by SDS-polyacrylamide gel electrophoresis were calculated to be 177, 121 and 90 kDa, respectively. SDS-polyacrylamide gel electrophoresis indicated that the lectin, MSL-1 was dimer in nature with two subunits held together by disulfide bonds. The other two lectins, MSL-2 and MSL-3 were tetramer in nature contained four non-identical subunits that were held together by nonionic hydrophobic interaction. The lectins agglutinated specifically rat red blood cells and galactose and galactose containing sugars were found to be the potent inhibitor of agglutination. The lectins were glycoproteins in nature with neutral sugar content of 5.6, 5.3 and 4.5% for MSl-1 MSL-2 and MSL-3, respectively. The sugar composition of the lectins was glucose and mannose for MSL-1 and galactose for both MSL-2 and MSL-3.

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