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Pakistan Journal of Biological Sciences
Year: 2004  |  Volume: 7  |  Issue: 10  |  Page No.: 1754 - 1757

Comparison of dsRNA Profiles of Sudanese Isolates of Epizootic Hemorrhagic Disease of Deer Virus

Salah M.M. Elamin, Tawfig E. Mohammed, Mohammed M. Salih, Mohammed A. Abdalla, Mohamed E.H. Mohamed, Rihab A. Omer, Abdelrahim Karrar and Imadeldin E. Aradaib    

Abstract: The double stranded RNA (dsRNA) genome segments of the Sudanese isolates of epizootic hemorrhagic disease of deer virus (EHDV) were analysed by agarose gel and sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (SDS-PAGE). EHDV serotype 1 and 2 are enzootic in the United States whereas EHDV-4 and an untyped isolate designated EHDV-318 are enzootic in the Sudan. The dsRNA genome segments profiles of the Sudanese EHDV serotypes 4 and EHDV-318 were compared with those of North American EHDV serotypes 1 and 2. Both systems (agarose gel and SDS-PAGE) showed 10 segments for each virus isolate, which is characteristic pattern of EHDV serogroup. The agarose gel showed identical genome profiles for all Sudanese and North American serotypes of EHDV serogroup. However, SDS-PAGE system was able to detect genetic variation between Sudanese and North American EHDV serogroups and among the Sudanese serotypes of EHDV. The results of this study suggested that, the agarose gel electrophoresis could be used as a supportive or complementary method to facilitate tentative diagnosis of EHDV infection in susceptible animal populations. In addition, the SDS-PAGE could also be used to detect genetic diversity among topotypes of EHDV serogroup from the African or North American continents.

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