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Pakistan Journal of Biological Sciences
Year: 2004  |  Volume: 7  |  Issue: 8  |  Page No.: 1371 - 1375

Multiple Shoot Regeneration Response of Recalcitrant Cotton (Gossypism hirsutum L.) Cultivar CIM-443

Rozina M. Ali, Tayyab Husnain, Syed Sarfraz Hussain, Nasir Mahmood and Sheikh Riazuddin    

Abstract: Induction of multiple shoots in Gossypium hirsutum L. variety CIM-443 has been achieved by using meristem and cotyledonary node as explants. Meristems of size 3-4 mm were excised from embryos isolated from seeds while cotyledonary nodes were cut from seven days old seedling and the mean shoot number per explant response for total of three experiments was maximum i.e., (6.72±0.79) and (4.92±0.67), respectively for both explants in MS medium supplemented with benzyl-amino purine (BAP) 1.0 mg L-1 + naphthalene acetic acid (NAA) 0.05 mg L-1. Shoot elongation was observed in MS medium amended with NAA 0.1 mg L-1. The percentage of shoots forming roots was maximum (79.16) in case of 1/2 MS supplemented with NAA 0.05 mg L-1. Rooted plantlets hardened in soil and normal boll formation observed.

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