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Pakistan Journal of Biological Sciences
Year: 2004  |  Volume: 7  |  Issue: 8  |  Page No.: 1360 - 1364

Coagglutination: A Rapid and Sensitive Assay Method for Detection of Vibrio cholerae O1 and O139 Serogroups Directly from Stool Specimens

Md. Monirul Islam, Md. Golam Kabir, A.F.M. Tariqul Islam and Md. Abdur Rakib    

Abstract: Using monoclonal antibodies (MAbs), the coagglutination test detected Vibrio cholerae O1 and O139 serogroups both in the bacterial culture and in the stool specimens. Detection of V. cholerae O1 and O139 from the cultures of different V. cholerae O1 and O139 strains and non-O1 and non-O139 strains showed a specificity of 100%, a sensitivity of 100% and a 100% positive predictive value. Among the 146 stool samples tested by the coagglutination test, 36 were culture positive, but 33 were coagglutination test positive showing a specificity of about 92%. All the culture negative samples were negative in the coagglutination test indicating a specificity of 100%.

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