Abstract: This study was conducted with varieties, inter-variety and inter-species hybrids of Brassica oil crop to determine genetic divergence. The divergence study indicated that parent, inter-variety and inter-species hybrids almost clearly forms five groups indicating that they are divergent and might be of value for future breeding programme. Based on the study on genetic divergence of the Brassica, the varieties having the performance and located in the distant clusters could be utilized for hybridization programme to develop desired high yielding varieties. The genotypes grouped together are less divergent than the ones which into different clusters.