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Pakistan Journal of Biological Sciences
Year: 2003  |  Volume: 6  |  Issue: 9  |  Page No.: 844 - 848

Agronomical and Physiochemical Characterization of Somaclonal Variants in Indica Basmati Rice

Sultana Rasheed, Tahira Fatima, Khurram Bashir, Tayyab Husnain and Shiekh Riazuddin    

Abstract: Seeds of three indica rice varieties B-370, B-2000 and Super basmati were sterilized and incubated on callus inducing medium (MS-Medium with 2 mgl-1 2,4-D) for 2, 4, 6 and 8 weeks and calli were obtained after somatic embryogenesis. After the incubation periods, calli were transferred to the regeneration medium. Regeneration efficiencies decreased from 98.5, 98 and 95% after two weeks incubation to 30, 34 and 24% after eight weeks incubation for B-370, Super basmati and B-2000 respectively. Some interesting patterns for adventitious root formation were also observed. Significant variation was observed within and among different cultivars and incubation periods for average number of tillers, average plant height, number of panicles per plant, panicle length and average yield per plant. Seven interesting somaclones were marked and scored for agronomic and physiochemical analysis. Physiochemical studies did not reveal any significant variation. Present studies clearly indicated that somaclonal variation can successfully be used for creating genetic variation for varietal improvement.

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