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Pakistan Journal of Biological Sciences
Year: 2003  |  Volume: 6  |  Issue: 4  |  Page No.: 334 - 335

Effect of Vegetative Inoculum on Submerged Citric Acid Fermentation by Aspergillus niger

Sikander Ali, Asad-ur- Rehman, Amna Ehsan, Ikram-ul- Haq and Javed Iqbal    

Abstract: The present investigation deals with the effect of vegetative inoculum on submerged citric acid fermentation by Aspergillus niger using stirred bioreactor. All fermentations were carried out following growth on 15% raw molasses sugars for 144 h. Ferrocyanide (200 ppm) was used to control the trace metals present in the molasses medium. The maximum production of citric acid was obtained when 1.0% vegetative inoculum was used. The dry cell mass and sugar consumption were 18.5 and 96.55 g l-1, respectively. The mycelia were intermediate round pellets in their morphology. The specific productivity (qp = 0.074±0.02a g g-1 cells h-1) was several folds higher than many other workers.

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