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Pakistan Journal of Biological Sciences

Year: 2003  |  Volume: 6  |  Issue: 24  |  Page No.: 1991 - 1995

Inter and Intra-specific Variation in SDS-PAGE Electrophoregrams of Total Seed Protein in Chickpea (Cicer arietinum L.) Germplasm

Rehana Asghar, Tayyaba Siddique and Muhammad Afzal


Genetic variation in germplasm has an important role in identification of varieties. Electrophoretic patterns of the protein fractions are directly related to the genetic background of the proteins and can be used to certify the genetic makeup. SDS-PAGE is a valid technique increasingly being utilized as an approach for species identification. Each variety or a group of varieties exhibit characteristic protein banding patterns. On the basis of these patterns they can be identified accordingly. Twenty-nine accessions of Cicer arietinum(Chickpea) germplasm were analysed for total seed protein profile using sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) to ascertain the extent of genetic variation and its geographical distribution. A considerable variation in protein banding pattern was observed which was localised to various geographical regions. Inter-specific variation was more as compared to intra-specific variation.

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