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Pakistan Journal of Biological Sciences
Year: 2003  |  Volume: 6  |  Issue: 22  |  Page No.: 1912 - 1916

Induction of Xylanase and Cellulase Genes from Trichoderma harzianum with Different Carbon Sources

Sibtain Ahmed, Qurrat-ul-Ain, Nighat Aslam, Saima Naeem, Sajjad-ur-Rahman and Amer Jamil    

Abstract: Xylanases and cellulases are industrially important enzymes obtained from various microorganisms. Trichoderma harzianum is a preferred microorganism for the production of complete xylanases and cellulases. E-58 strain of the fungus was grown in different media including Enzyme Production Medium (EPM) and Vogel`s medium with different carbon sources like xylan, glucose and CMC to get the optimal production of xylanases and cellulases. It was found that glucose repressed the synthesis of these enzymes whereas xylan and CMC produced these enzymes in substantial amounts. Maximal activity of cellulases and xylanases was marked at pH 5.5 and at temperature 28°C. The fungus was grown for 5 days at 120 rpm in orbital shaker. Maximum enzyme activity of xylanase was found to be 1.209 IU mL-1 whereas maximum activity of cellulases (exoglucanase, endoglucanase and β-glucosidase) was found to be 2.764, 14.4 and 1.1209 IU mL-1 respectively.

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