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Pakistan Journal of Biological Sciences
Year: 2003  |  Volume: 6  |  Issue: 14  |  Page No.: 1250 - 1252

Induction of Haploid Rice Plants Through in vitro Anther Culture

Sharmin Shahnewaz, M.A. Bari, N.A. Siddique, N. Khatun, M.H. Rahman and M.E. Haque    

Abstract: Anthers of three rice cultivars having late uninucleate microspores were studied for induction of haploid rice plants. Anthers were cultured on Z2 medium supplemented with various combinations and concentrations of auxins and kinetin (cytokinin). The best callusing from cultured anthers obtained in 2 medium containing 2,4-D 2 mg l-1, NAA 2.5 mg l-1 and kin 0.5 mg l-1. For regeneration of haploid plantlets anther derived calli were transferred to modified MS medium enriched with kin 1 mg l-1, NAA 1 mg l-1, BAP 1.0 ml l-1 and incubated at 25±1OC in light. BRRI Dhan-29 produced both green and albino plants while BR-3 produced green plants only. Green plantlets obtained from BRRI Dhan-29 were examined and found to be 69.2% haploid plants.

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