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Pakistan Journal of Biological Sciences

Year: 2002  |  Volume: 5  |  Issue: 9  |  Page No.: 956 - 958

Callus Induction from Seeds of Zea mays Var. EV-2097

S.M.S. Naqvi, T. Yasmin, H. Rashid, Z. Chaudary and A. Quraishi


The present study was conducted on maize, with the aim to work out a suitable surface disinfecting procedure for seeds and to know the best concentration of 2,4-dichlorophenoxyacetic acid (2,4 -D) for callus induction in N6 medium using var. EV-2097. Better disinfection of seeds was achieved with 30 minutes Clorox treatment (86 %). Maximum callus induction (47 %) was recorded with 2 mg l-1 followed by 3 mg l-1 (18 %) and 1 mg l-1 2,4 -D (16 %).

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