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Pakistan Journal of Biological Sciences
Year: 2002  |  Volume: 5  |  Issue: 6  |  Page No.: 679 - 682

Initiation and Cryopreservation of Cell Suspension of Rice Basmati Varieties

Tahira Fatima, Asad Jan, Tayyab Husnain and Sheikh Riazuddin    

Abstract: Efforts were made to optimize tissue culture conditions for quick establishment of cell suspension and simple procedure for the cryopreservation of embryogenic cells suspension cultures of three rice varieties. Suspension cultures were initiated from friable, globular embryogenic calli in MS/R2 media supplemented with 2mg/l 2,4-D. These suspension cultures were regenerated and cryopreserved. Both MS and R2 media supplemented with 2mg/l 2,4-D were quiet suitable for developing cell suspension. All the three varieties gave compact light yellow calli on MMS medium. The regeneration frequency was 55% on average for 10-14 weeks old cell suspension. Vigorous regeneration was observed on MS containing sorbitol. Post thaw cell viability varied from variety to variety. No significant difference in viability was found in varieties cryopreserved for different periods. In conclusion cryopreservation can be used to preserve cell suspension line for a reasonable time. It can easily be used on culture medium or liquid R2 medium.

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