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Pakistan Journal of Biological Sciences
Year: 2002  |  Volume: 5  |  Issue: 6  |  Page No.: 659 - 661

In vitro Clonal Propagation of Sugarcane (Saccharum officinarum) Variety Isd 31

M.Z. Karim, R. Alam, R. Baksha, S.K. Paul, M.A. Hossain and A.B.M. Mafizur Rahman    

Abstract: Leaf sheath were used as explants to induce callus on modified MS media supplemented with 2, 4-D as growth regulators. Different concentrations of BAP, IBA, NAA and IAA were used to regenerate shoots and a concentration of 1.0 mg/l BAP and 0.5mg/l IBA was found superior in the optimum production of multiple shoots. NAA (5mg/l) showed best performance in the production of roots. The plantlets were successfully transferred to soil with 70 percent survivability.

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