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Pakistan Journal of Biological Sciences
Year: 2002  |  Volume: 5  |  Issue: 1  |  Page No.: 57 - 60

Genetic Diversity of Streptococcus uberis Strains Isolated from Clinical and Subclinical Cases of Bovine Intramammary Infections in Malaysia

Md. Firoz Mian, Abdul Rahim Mutalib, Mohd Azmi Mohd Lila, Abdul Aziz Saharee and Nadzri Salim    

Abstract: Random amplified polymorphic DNA (RAPD) analysis was employed on twenty-four Streptococcus uberis isolates from cases of clinical and sub-clinical mastitis from five smallholder dairy herds in Malaysia. Three ten-mer primers namely OPA-01, OPA-05 and OPA-07 were selected out of a 20 primers set. A high degree of genetic polymorphism was revealed among the isolates of S. uberis by RAPD-PCR. Fourteen distinct RAPD fingerprint patterns were generated with primer OPA-05, while primers OPA-01 and OPA-07 produced 12 and 11 RAPD patterns respectively. The discriminatory power of this technique could be further improved by the parallel use of several primers. Most of the isolates were genetically different strains, however, identical patterns were noted among different cows within the same farm or from different cows from different farming regions. These findings indicate that RAPD analysis is a rapid and reproducible method for molecular sub-typing of bovine S. uberis isolates and therefore, represents a powerful tool for epidemiological studies.

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