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Pakistan Journal of Biological Sciences
Year: 2001  |  Volume: 4  |  Issue: 6  |  Page No.: 714 - 717

Growth, Ochratoxin A Production and Some Metabolic Activities of Aspergillus ochraceus at Different NaCl Concentrations

M. A. Metwally and M. A. Hefnawy    

Abstract: The growth of Aspergillus ochraceus was decreased with increasing NaCl concentration. The decreasing ratio of ochratoxin A by NaCl ranged from 25 to 74% at 5 and 15% NaCl respectively. The total soluble protein increased to 37% at 15% NaCl. The total lipids decreased only at 15% NaCl. The myristic and palmitic acids represented the highest concentrations of saturated fatty acids while their concentrations decreased with increasing NaCl. Whereas, 10% NaCl increased the concentrations of the unsaturated fatty acids (oleic and linoleic) and decreased palmitoleic acid. On the other hand 15% NaCl increased linolenic and decreased palmitoleic and oleic acid concentrations. Most of the detected phospholipids increased with increasing NaCl concentrations in the growth medium up to 10%, whereas they decreased at 15% NaCl. Phosphatidyl glycerol, phosphatidyl ethanolamine and phosphatidic acid were the major phospholipids.The genomic DNA of the control and the five NaCl treated samples showed a common band at higher molecular weight.

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