Abstract: Growing on test was conducted in aluminum trays and photographic cassette holders to determine the germination and seed transmission of jute leaf mosaic agent using five Corchorus capsularis cultivars. The test cultivars (D-154 and CVL-1) were grown in successive two years in a net house. Seeds collected from early-infected plants were used in the second year. Significantly higher percentage of seed transmission was obtained in the second year. Among the five test cultivars grown in two agro- ecological zones CVL-1 performed the best but none of the cultivars was free from leaf mosaic disease infection. Agro-ecological factors influenced the occurrence of the disease.