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Pakistan Journal of Biological Sciences
Year: 2001  |  Volume: 4  |  Issue: 9  |  Page No.: 1165 - 1169

Protective Efficacy of Subunit Antigens Isolated from In vitro Mid Gut Cells of Boophilus microplus

Masood Akhtar and C.S. Hayat    

Abstract: Protein subunit (PS) and lipopolysaccharides complex subunit (LPCS) antigens were extracted from the in vitro cultured mid gut cells of Boophilus (B.) microplus. PS and LPCS vaccines with and without adjuvant was prepared and injected in buffaloes. Their efficacy was evaluated on the basis of cellular, humoral and challenge responses. There was a significant difference (P<0.05) in cellular, humoral and challenge responses between the two subunit vaccines. The vaccines with adjuvant gave significantly higher cellular, humoral and challenge responses compared to those without adjuvant. The per cent protection and damage of B. microplus in PS vaccines with and without adjuvant was 75.79 and 61.56; and 44.56 and 42.78 per cent, respectively while in LPCS vaccines with and without adjuvant was 89.25 and 73.79; and 76.25 and 64.16 per cent, respectively. The results of the current study confirm the role of protein and carbohydrates as a protective antigen or as a factor for specificity of these antigen.

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