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Pakistan Journal of Biological Sciences
Year: 2001  |  Volume: 4  |  Issue: 9  |  Page No.: 1129 - 1132

Purification, Characterization and Effect of Physico-chemical Agents on Stability of Polyphenoloxidase from Sajna (Moringa oleifere L.) Leaves at Mature Stage

Shahanaz Khatun, Nurul Absar and M. Ashraduzzaman    

Abstract: Polyphenoloxidase (PPO), a highly active oxidative enzyme in Sajna leaves at mature stage was purified by ion exchange chromatography on DEAE-cellulose followed by CM-cellulose. The purified enzyme was monomer in nature and its molecular weight was estimated to be 56,000 and 55,500 by gelfiltration and SDS-PAGE respectively. The enzyme appeared to be a single polypeptide chain as revealed by SDS-PAGE either in presence or absence of reducing agent. The enzyme has following characteristics : optimum pH 6.2, optimum temperature 32°C, Km value 0.047M against catechol as substrate and maximum absorption 279 nm. The activity of the enzyme was enhanced by Ca2+ and Cu2+ but its activity lost completely in presence of ascorbic acid, EDTA, KCN and NaHSO3.

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