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Pakistan Journal of Biological Sciences

Year: 2000  |  Volume: 3  |  Issue: 6  |  Page No.: 911 - 914

Multidrug Resistant Gene(S) Harboring On A 20 Kb Plasmid In Salmonella typhi That Causes Typhoid-Enteric Fever

Md. Abdul Hye Khan, Asif Hasan Chowdhoury, M. Ashik Mosaddik and M. Shajahan


Ten multidrug resistant Salmonella typhi were isolated from the blood and stool samples of clinically suspected patients suffering from typhoid fever. To see whether the drug resistance phenomenon was plasmid mediated, one of these resistant isolates was used for the plasmid analysis and a single 20 kb plasmid was found. This 20 kb plasmid was transferred to a sensitive E. coli LE 392 and after the plasmid transfer experiment E. coli LE 392 acquired resistance against those previously used antibiotics. Plasmid analysis of the transformed E. coli LE 392 further exhibited that it harbored a single 20 kb plasmid corresponding to that of the wild S. typhi which further confirmed that the 20 kb plasmid was carrying the gene(s) responsible for the multidrug resistance in Salmonella typhi.

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