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Pakistan Journal of Biological Sciences

Year: 2000  |  Volume: 3  |  Issue: 4  |  Page No.: 621 - 622

Genetic Relatedness of Vibrio cholerae O1 and non-O1 by ERIC Polymerase Chain Reaction and Pulsed-Field Gel Electrophoresis

Yuherman, Son Radu, Gulam Rusul, Lum Keang Yeang, Ooi Wai Ling and Jamal Khair

Abstract

DNA fingerprinting by PCR amplification of enterobacterial repetitive intergenic consensus (ERIC) and pulsed-field gel electrophoresis (PFGE) were used to compare environmental and clinical isolates of Vibrio cholerae O1 and non-O1. All the V. cholerae O1 and non-O1 isolates were typable using ERIC PCR. Though PFGE generated banding patterns to discriminate the isolates into twelve fingerprints, eight isolates were untypable by PFGE due to consistent degradation of the bacterial DNA. Based on the dendrogram generated from ERIC-PCR method, three of the clinical isolates (C1, C2 and C3) were closely related to environmental isolates (E6 or E10). The results indicate that ERIC-PCR is a very discriminative and efficient method for studying genetic diversity of V. cholerae isolates.

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