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Pakistan Journal of Biological Sciences
Year: 2000  |  Volume: 3  |  Issue: 12  |  Page No.: 2232 - 2237

Inducible Tolerance to Heavy Metals in Air Borne Bacteria

Nuzhat Ahmed, Fehmida Fasim, Mohd. Arif and Nazia Jamil    

Abstract: Airborne bacteria were isolated from three polluted sites around Karachi. Among 30 isolates CMG 921 (Proteus mirabllis), 922 (Pseudomonas putida), 923 (Pseudornonas pseudomallei) and 924 (Yersinia aldovae) were selected and studied. All these strains belonged to Gaddani and were resistant to multiple metals indicating the high level of pollution in Gaddani. These strains were sensitive to most of the antibiotics except ampicillin. It was found that the metal resistant did not involve the outer membrane permeability however the resistance to copper and nickel was inducible. A single band of plasmid DNA of 2 kb was observed in CMG 921 and CMG 922 which have shown the same level of metal resistance. This has suggested that there is a horizontal transfer of plasmid pAN10 from one strain to another and perhaps the metal resistant determinants present on this plasmid.

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