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Pakistan Journal of Biological Sciences
Year: 2000  |  Volume: 3  |  Issue: 11  |  Page No.: 1981 - 1984

Analytical Investigation of Fluorescent Complexes of Valine-8-hydroxyquinoline and Valine-8-hydroxyquinaldine in Aqueous Phase Using Spectroscopic Techniques

M.A. Jakhrani, T.G. Kazi, G.H. Kazi, M.H.S. Wattoo and Farooq Anwar    

Abstract: A new method has been developed by preparing complexes involving condensation of amino acids with 8-hydroxyquinoline and 8-hydroxyquinaldine. The products so obtained are being investigated for identification and quantitative estimation of amino acids using different spectroscopic techniques including fluorescence activity of newly synthesized products. The method adopted in our laboratories is rapid, versatile, reproducible and provides excellent results for adoption by analytical, agricultural and biomedical laboratories to estimate amino acids and metals in composite matrix at economical costs. 8-hydroxyquinoline (Oxine) and 2-methyl-8-hydroxyquinoline (8-hydroxyquinaldine) condensed with valine produced fluorescent complexes. The complexes have been investigated for identification and quantitative estimation of amino acids. By this method identification of amino acids in nano mole quantities has become possible by fluorescence activity of valine-8-hydroxyquinoline and valine-8-hydroxyquinaldine complexes involving different excitation and emission wave lengths. This fluorescence activity of complexes is 100 to 1000 times higher than assay method involving ninhydrin and amino acid analyzer.

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