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Pakistan Journal of Biological Sciences
Year: 2000  |  Volume: 3  |  Issue: 8  |  Page No.: 1313 - 1314

Comparative Immune Response of Formalin Inactivated and Binnary Ethyleneimine Inactivated Angara Disease Vaccines

Masood Akhtar, Rehan Ahmed, C. S. Hayat, I. Hussain and M. Ashfaque    

Abstract: Immune response of formalin inactivated and binnary ethyleneimine inactivated Angara disease (hydropericardium) vaccines were compared using indirect hemagglutination (IHA) test. A four fold increase in IHA antibody titre was recorded with binnary ethyleneimine (BEI) inactivated vaccine. Agar gel diffusion test gave a strong precipitation line with serum of chicks vaccinated with BEI inactivated vaccine whereas a weak precipitation line was observed with serum from chicks vaccinated with formalin inactivated vaccine. The results of challenge test were promising and no adverse effects were seen in vaccinated chicks. BEI as inactivating agent produced antigenically superior vaccine.

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