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Pakistan Journal of Biological Sciences

Year: 1999  |  Volume: 2  |  Issue: 3  |  Page No.: 984 - 987

Optimization of Different Media for Plant Regeneration from Callus Culture of Indica Rice (Oryza sativa) Genotype DM. 25

Zafar Iqbal Khan, Altaf Hussain and Muhammad Sadiq


Different Media were optimized for plant regeneration from callus initiated from mature embryos of Indica rice on media with various levels of 2,4-D alone and in combination with various concentrations of Benzyle adenine. Plant regeneration was achieved in both MS and N6 media with and without various levels of growth regulators. In addition to green plants, production of albino plants was also observed in all the media tested for regeneration. The composition of each Callus induction and regeneration media was found to be responsible for the recovery of higher number of plants.

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