Isolation and Screening of Amylolytic and Pectinolytic Fungi from Soil
Zafar Iqbal Khan
Dr. Ejaz Rasul
Eleven fungal species namely Aspergillus niger, Aspergillus flavus, Aspergillus terreus, Penicillium spp. Alternaria alternata, Fusarium pallidoroseum, Fusarium oxysporum, Aspergillus fumigatus, Rhizopus stolonifers, Fusarium moniliforme and Cephalosporiopsis sp. were isolated. Out of these species, first 7 were amylolytic and last 4 were pectinolytic. These species were employed for the production of amylases and pectinases respectively. Among the amylolytic species the glucoamylase activity (IU/ml) in surface cultures was found in the order; 17.4 ,15.20,14.0,9.6,5.0,4.50, and 4.00 for A. niger, A. flavus, A. terreus, Penicillium sp., F. pallidoroseum, F. oxysporum and Alternaria alternata respectively and glucoamylase activity in submerged cultures was found for the same fungal strains in the order; 10.00, 9.00, 6.80, 6.20, 5.0, 3.88 and 3.50 units/ml respectively. Among the pectinolytic fungi the pectinase activity was found only in the surface culture in the order; 15.30,12.00,10.20, and 8.00 units/ml for Rhizopus stolonifers, Aspergillus fumigatus, Fusarium moniliforme and Cephalosporiopsis sp. respectively.
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