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Pakistan Journal of Biological Sciences
Year: 1999  |  Volume: 2  |  Issue: 3  |  Page No.: 834 - 837

Elimination of Interferences of Organic Nitrogen Compounds by Dialysis Inclusion with Technicon AutoAnalyzer II in Ammonium-N Determination

Muhammad Ayyaz Khan and T.H. Flower    

Abstract: The dialysis system was included in the main manifold for ammonium-N determination of the Technicon AutoAnalyzer II. Studies using this system indicated that only traces of NH4-N were present as impurities in organic nitrogen compounds such as urea, aspartic acid, lysine and histidine. Positive and negative interferences in the NH4-N determination were attributed to hydrolysis or participation of the organic nitrogen compounds in the colorimetric reaction. These positive and negative interferences were eliminated by gas dialysis. The level of random variability measured with dialysis was consistant with random variability due to solution preparation and to the system complexity. The dialysis system was further developed for the determination of NH4-N in Kjeldhal digests. Dialysis use with Technicon AutoAnalyzer II did not disturb the precision of the system.

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