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Pakistan Journal of Biological Sciences
Year: 1999  |  Volume: 2  |  Issue: 3  |  Page No.: 829 - 833

Development of a Gas Dialysis System for NH4-N Determination

Muhammad Ayyaz Khan and T.H. Flower    

Abstract: The development of a gas dialysis system proved that ammonium-N recovery was inversely proportion to acceptor flow rate. The relationship between amonia trapping and acceptor flow rate was considered a crucial parameter and the best flow rate for donor and acceptor streams was chosen for the analysis of water and extract samples. The Technicon silicon gas dialysis membrane showed very low ammonia transference. Polytetrafluoroethene (PTFE) membranes allowed higher amounts of ammonia to be transferred through them. Recovery of ammonia using the standard 6 inch dialyser was 56.7 percent. Analysis at 20 samples per hour showed adequate peak separation and good peak shapes. The standard deviation of the results was between 0.002 and 0.004 for a 1 mg l-1 ammonium-N solution. Calibration curves were linear (y=4.14+15.7x, r2=0.99 and y=5.11+42.82x, r2=0.98). Hence, the developed system was sufficiently precise and reliable for its use in NH4-N determination.

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