Abstract: Field experiments on two Alfisols at different locations were conducted to study the response of Westar and CON-I varieties of Brassica napus to S application (0 and 37 kg ha^-1) as ammonium sulphate, gypsum and single superphosphate. Various S fertilizers, Brassica varieties and location of the experiment had a significant (p<0.05) main and interactive effect on the grain and oil yield of Brassica. Different S-amendments in increasing grain yield of Brassica followed the order: ammonium sulphate > gypsum > single superphosphate. Westar was a better grain-yielding and a more S-responsive variety of Brassica than CON-I. Both grain and oil yield of Brassica were higher on a site (Fateh Jang) containing low CaCO₃ and high plant-available P. Sulphur concentration in seed was found in strong antagonism with seed P (r= -0.79, n= 16).