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Pharmaceutical Biology

Year: 2011  |  Volume: 49  |  Issue: 9  |  Page No.: 962 - 969

Antiproliferative effects of extracts from Iranian Artemisia species on cancer cell lines

Shahrzad Zamanai Taghizadeh Rabe, Mahmoud Mahmoudi, Ali Ahi and Seyed Ahmad Emami


Objective: Different species of Artemisia (Asteraceae) have shown to exhibit antitumor activity. The aim of this study was to identify the antiproliferative effect of some Artemisia species from Iran on cultured human cancer cells. Materials and methods: Methanol, ethyl acetate, dichloromethane and n-hexane extracts from aerial parts of seven species of Artemisia were prepared and their antiproliferative effects on four cancer (AGS, HeLa, HT-29 and MCF-7) and normal cell line (L929) were determined. Different concentrations of extracts were added to cultured cells and incubated for 72 h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was employed to assess the cell viability. Results: Different extracts exert various growth inhibitory effects. In case of AGS cells, dichloromethane and methanol extracts of A. ciniformis Krasch. & Popov ex Poljak. (IC50 values: 35 and 60 μg/ml, respectively) showed the highest growth inhibitory effects. HeLa cells were more sensitive to both A. diffusa Krasch. ex Poljak. dichloromethane (IC50 value: 71 μg/ml) and A. ciniformis ethylacetate (IC50 value: 73 μg/ml) extracts. Dichloromethane extracts of A. diffusa, A. santolina Schrenk and A. ciniformis (IC50 values: 42, 91 and 94 μg/ml, respectively) exhibited more inhibition on HT-29 cells in comparison to other extracts. MCF-7 cells were best inhibited by A. ciniformis dichloromethane (IC50 value: 29 μg/ml) and A. vulgaris L. ethyl acetate (IC50 value: 57 μg/ml) extracts. Discussion and conclusion: This study shows the antiproliferative effects of Artemisia extracts on malignant cell lines. Artemisia could be also considered as a promising chemotherapeutic agent in cancer treatment.

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