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Molecular Endocrinology
Year: 2009  |  Volume: 23  |  Issue: 6  |  Page No.: 759 - 771

The Orphan Nuclear Receptor ROR{alpha} Restrains Adipocyte Differentiation through a Reduction of C/EBP{beta} Activity and Perilipin Gene Expression

N Ohoka, S Kato, Y Takahashi, H Hayashi and R. Sato    

Abstract:

The nuclear receptor-type transcription factor retinoic acid receptor-related orphan receptor (ROR) is a multifunctional molecule involved in tissue development and cellular function, such as inflammation, metabolism, and differentiation; however, the role of ROR during adipocyte differentiation has not yet been fully understood. Here we show that ROR inhibits the transcriptional activity of CCAAT/enhancer-binding protein β (C/EBPβ) without affecting its expression, thereby blocking the induction of both PPAR and C/EBP, resulting in the suppression of C/EBPβ-dependent adipogenesis. ROR interacted with C/EBPβ so as to repress both the C/EBPβ-p300 association and the C/EBPβ-dependent recruitment of p300 to chromatin. In addition to the inhibitory effect on C/EBPβ function, ROR also prevents the expression of the lipid droplet coating protein gene perilipin by peroxisome proliferators-activated receptor (PPAR), acting through the specific mechanism of its promoter. We identified a suppressive ROR-responsive element overlapping the PPAR-responsive element in the perilipin promoter and verified that ROR competitively antagonizes the binding of PPAR. ROR inhibits PPAR-dependent adipogenesis along with the repression of perilipin induction. These findings suggest that ROR is a novel negative regulator of adipocyte differentiation that acts through dual mechanisms.

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